e-Health startups are at the forefront of healthcare innovation, offering solutions aimed at improving access to care, optimizing patient support, and introducing new organizational models. Strategic partnerships with research institutions, scientific societies, and patient organizations, as well as diversifying funding sources (public, private, fundraising, and grants), are at the core of their development strategy. e-Health startups face numerous challenges to establish themselves in a competitive sector, meet strict regulatory standards, and prove the effectiveness of their solutions in line with user and payer expectations. Demonstrating the added value of solutions, identifying adequate funding, integrating technologies into existing systems, and ensuring the security of patient data are key challenges to ensure their sustainable development.